The type of lysis buffer used depends on the cell source (tissue culture, plant, bacteria, fungi, etc.), and whether the cells are in a structure and the type of structure. There are a number of different types of lysis buffer for protein extraction. The protocols and lysis buffers for proteins are generally different from the lysis buffers used for nucleic acids. The type of lysis buffer used depends on the types and source of cells, the desired final molecule or structure, and the level of their functionality. In most purification protocols for nucleic acids, proteins, membranes, or organelles, the first step is usually cell lysis. Cell Lysis: The First Step in the Extraction of Molecules and Structures
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